kousa) than in the non-bracted species ( C. Cross species transfer was higher in the big-bracted dogwood species ( C. The number of alleles detected ranged from 3 to 10 and observed heterozygosity was 0.05 to 0.67. Seventy-five of the loci amplified and 29 were polymorphic, from which 9 of the most poly¬morphic loci were selected and tested using 22 individuals representing 6 dogwood species and an interspecific hybrid. To demonstrate the usefulness of this EST resource for marker development, primers were designed for 2,998 of the EST-SSRs of which 94 were tested for amplification of loci in √ppalachian Spring, ∜herokee Brave, and two F1 breeding lines. The 1,621,644 reads were assembled into 63,029 unique sequences, comprising 42,946 isotigs (26,627 isogroups) and 20,083 singletons.Īlso, 5,670 expressed sequence tags (EST) simple sequence repeats (SSRs) were identified from the data set. To obtain more information on the flowering dogwood genome, a single custom, normalized library for Roche/454 sequencing was prepared and sequenced for bract (∜herokee Brave) and leaf (√ppalachian Spring and ∜herokee Brave) tissue.
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